Scientists tested a new commercial horse feed designed to help with both hydration and nutrition to see how it affects the helpful bacteria living in horses’ stomachs. Using a lab setup that mimics a horse’s digestive system, they compared this special feed added to two different types of regular horse diets. The results showed that the new feed didn’t cause any problems and actually changed the bacteria in ways that could be beneficial, especially when horses were eating a mix of hay and grain. This research helps horse owners and veterinarians understand how different feeds affect the invisible ecosystem of bacteria that keeps horses healthy.

The Quick Take

  • What they studied: Whether a new fiber-based commercial feed changes the bacteria living in a horse’s hindgut (back part of the stomach) in helpful or harmful ways
  • Who participated: This wasn’t a study with live horses. Instead, scientists used laboratory equipment that simulates how a horse’s digestive system works, testing the feed three times with different combinations
  • Key finding: The special feed didn’t cause any negative effects on the bacteria. When horses ate a mix of hay and grain, adding the special feed actually changed the bacterial community in ways that appear beneficial
  • What it means for you: Horse owners might have a safe new feeding option that could support better digestive health, but more research with actual horses is needed before making feeding changes. Talk to your veterinarian before trying new feeds

The Research Details

Scientists created a laboratory model of a horse’s hindgut—the part of the stomach where most digestion happens. This artificial system included the right temperature, bacteria, and conditions to mimic what happens inside a real horse. They tested four different feeding scenarios: hay-only diets with and without the special feed, and hay-plus-grain diets with and without the special feed. Each combination was tested three separate times to make sure results were consistent. Every day, the artificial system was fed like a real horse would be, and special saliva was continuously added to match natural digestion.

Testing in a laboratory first is important because it’s safer and faster than testing with live horses. It lets scientists understand exactly how a feed affects the bacteria without any risk to animals. The hindgut bacteria are crucial for horse health—they help break down food, produce energy, and protect against disease. Understanding how feeds change these bacteria helps create better nutrition products.

This study was well-designed with careful controls and repeated testing. However, because it used a laboratory model rather than real horses, the results need to be confirmed with actual animals before making strong recommendations. The study was published in a respected equine veterinary journal, which means experts reviewed the work. The small number of test repetitions (three times) is typical for this type of laboratory research but means results should be interpreted cautiously.

What the Results Show

The special fiber-based feed showed no harmful effects on the bacterial community or fermentation process in either diet type tested. This is important because it means the feed appears safe to use. When horses ate the hay-and-grain mixture (the more common feeding approach), adding the special feed changed the bacterial community in measurable ways. The fermentation profile—which shows how well bacteria are breaking down food—was different depending on whether horses ate hay alone or hay with grain, but adding the special feed didn’t negatively impact this process. The researchers found that the type of base diet (hay only versus hay plus grain) had a bigger effect on bacteria than adding the special feed, suggesting that what horses eat as their main diet matters more than supplements.

The study showed that different basal diets created different bacterial communities, which confirms what scientists already suspected—that a horse’s main diet is the biggest factor shaping gut bacteria. The special feed’s effects were most noticeable in the hay-and-grain diet group, suggesting it might work better as a supplement to mixed diets rather than hay-only diets. No unexpected or concerning changes in fermentation were observed with any combination tested.

Previous research has shown that horse health depends heavily on gut bacteria, and diet is the main thing that changes these bacteria. This study adds to that knowledge by testing a specific commercial product. While many horse feeds claim to improve digestion, few have been scientifically tested for their effects on gut bacteria. This research fills that gap for one particular product and suggests the approach could be used to test other feeds.

The biggest limitation is that this was laboratory research, not a study with real horses. A horse’s actual digestive system is more complex than the laboratory model, and living horses have individual differences that the model can’t capture. The study only tested one commercial feed product, so results don’t apply to other brands. The researchers didn’t specify exactly how many bacterial samples were analyzed, which makes it harder to assess the strength of the findings. Additionally, the study measured short-term effects (24 and 48 hours), so we don’t know what happens with long-term feeding. Finally, the study didn’t test the feed’s effects on actual horse health outcomes like weight, energy, or disease resistance.

The Bottom Line

Based on this research, the special fiber-based feed appears safe for horses and may offer benefits, particularly for horses eating mixed hay-and-grain diets. However, confidence in this recommendation is moderate because the study was done in a laboratory, not with real horses. Before adding this feed to your horse’s diet, consult with your veterinarian, especially if your horse has digestive issues or is on a special diet. More research with actual horses is needed to confirm these laboratory findings.

Horse owners feeding mixed diets (hay plus grain) might benefit most from this research. Veterinarians interested in equine nutrition and digestive health should be aware of this product’s safety profile. Horse breeders and trainers managing multiple horses could consider this as a potential supplement. People should NOT use this as a substitute for veterinary care if their horse has digestive problems. Horses with specific medical conditions should only receive new feeds under veterinary supervision.

Because this was a laboratory study, we can’t predict how long it would take to see benefits in real horses. If a veterinarian recommends trying this feed, allow at least 2-4 weeks to observe any changes in your horse’s condition, energy level, or coat quality. Some horses may respond faster than others. If no improvements are seen after a month, discuss alternatives with your veterinarian.

Want to Apply This Research?

  • If using this feed, track your horse’s daily digestive health by noting stool consistency (1-5 scale: 1=very loose, 5=very hard), energy level during exercise, and coat shine quality. Record these observations 3-4 times weekly to spot patterns over time.
  • Start by introducing the special feed gradually over 7-10 days, mixing it with current feed in increasing amounts. Use the app to log the introduction schedule and any changes you notice in your horse’s behavior, appetite, or digestion. This helps identify if any reactions occur during the transition.
  • Create a long-term tracking dashboard showing weekly digestive health scores, exercise performance, and overall condition. Compare data from before starting the feed to 4, 8, and 12 weeks after introduction. Share monthly summaries with your veterinarian to ensure the feed is working well for your specific horse.

This research describes laboratory findings only and has not been tested in living horses. Before making any changes to your horse’s diet or feeding program, consult with an equine veterinarian. This information is not a substitute for professional veterinary advice, diagnosis, or treatment. Horses with existing digestive problems, medical conditions, or those taking medications should only receive new feeds under direct veterinary supervision. Individual horses may respond differently to dietary changes. If your horse shows signs of digestive upset, decreased appetite, or other health concerns after dietary changes, contact your veterinarian immediately.

This research translation is published by Gram Research, the science division of Gram, an AI-powered nutrition tracking app.

Source: An investigation into the impact of the inclusion of a commercial fiber-based feed, designed for both hydration and nutrition, on the equine hindgut microbiomes.Journal of equine veterinary science (2026). PubMed 41865910 | DOI