Researchers tested whether green propolis, a natural substance made by bees, could work as well as salinomycin (a common antibiotic used in sheep feed) when sheep eat high-grain diets. This matters because antibiotics in animal feed are becoming a concern worldwide—overuse can create resistant bacteria that are harder to treat. The study explored whether this natural alternative could help farmers raise healthier sheep while reducing antibiotic use. Green propolis contains compounds that may help with digestion and animal health, making it a potentially safer option for both animals and people.

The Quick Take

  • What they studied: Can green propolis (a natural bee product) do the same job as salinomycin (an antibiotic) in sheep feed when sheep eat lots of grain?
  • Who participated: The study involved sheep on high-grain diets, though specific details about the number of animals and their characteristics weren’t provided in the available information.
  • Key finding: Green propolis appears to work similarly to salinomycin in supporting sheep health on grain-heavy diets, suggesting it could be a natural replacement for this antibiotic.
  • What it means for you: If you care about reducing antibiotic use in food production, this research suggests natural alternatives exist. However, more testing is needed before farms widely switch to this approach.

The Research Details

Researchers conducted an experiment comparing two groups of sheep: one group received salinomycin (the standard antibiotic additive) in their high-grain feed, while another group received green propolis instead. They then measured how well each group performed in terms of health, digestion, and growth. This type of comparison study helps determine whether a new substance works as well as an established one. By testing both groups under similar conditions, researchers could fairly evaluate whether the natural alternative was effective.

This research approach is important because it directly answers a practical question farmers face: can we use natural products instead of antibiotics? The study design allows researchers to see real-world effects on actual animals eating realistic diets. Understanding whether natural alternatives work helps address growing concerns about antibiotic resistance—a major public health issue where bacteria become immune to medicines we rely on.

The study was published in a peer-reviewed journal focused on animal health in tropical regions, which suggests it met scientific standards for publication. However, the full details about sample size and statistical analysis weren’t available in the provided information, which limits our ability to fully assess the study’s strength. Readers should note that more information about how many sheep were studied and how results were analyzed would help confirm the reliability of these findings.

What the Results Show

The research suggests that green propolis performed similarly to salinomycin when added to high-grain sheep diets. This is significant because it indicates that a natural product could potentially replace a synthetic antibiotic without compromising animal health or performance. Sheep receiving green propolis appeared to maintain comparable growth rates and digestive function compared to those receiving the standard antibiotic treatment. This finding is encouraging for farmers looking for alternatives to conventional antibiotics in animal feed.

The study likely examined additional outcomes such as feed efficiency (how well sheep convert feed into body weight), digestive health markers, and overall animal well-being. These secondary measures help paint a complete picture of whether green propolis truly matches salinomycin’s benefits. Understanding these additional effects is important for farmers considering switching to natural alternatives, as they need to know the full impact on their animals’ health and productivity.

This research builds on growing interest in natural alternatives to antibiotics in livestock farming. Previous studies have shown that propolis and other bee products have antimicrobial and anti-inflammatory properties that could benefit animal health. This study specifically tests whether those properties translate to real-world effectiveness in sheep on grain-heavy diets, filling a gap in the research about practical applications of these natural products.

The study’s main limitation is that specific details about sample size and statistical methods weren’t provided, making it harder to assess how confident we should be in the results. Additionally, the research focused specifically on sheep eating high-grain diets, so results may not apply to sheep on other types of feed or to other livestock species. More research with larger groups of animals and longer observation periods would strengthen these findings.

The Bottom Line

Based on this research, green propolis shows promise as a potential replacement for salinomycin in sheep feed (moderate confidence level). However, farmers should not make immediate changes without consulting with veterinarians and waiting for additional confirmation studies. More research is needed to establish optimal dosages, cost-effectiveness, and long-term safety.

This research matters most to sheep farmers, veterinarians, and people concerned about antibiotic use in food production. It’s particularly relevant for farmers in tropical regions where propolis is more readily available. Consumers interested in reducing antibiotic use in their food supply should also find this encouraging, though individual farms’ practices vary widely.

If green propolis were adopted on farms, benefits would likely appear within weeks as it affects digestion and nutrient absorption. However, full assessment of long-term health benefits and any potential drawbacks would require months to years of observation across multiple farms.

Want to Apply This Research?

  • If you raise sheep or manage a farm, track weekly feed costs and animal weight gain when comparing antibiotic-based feed to green propolis-based alternatives. Record any changes in animal health indicators like digestion quality and overall vitality.
  • Start by consulting with a veterinarian about sourcing green propolis and creating a small test group of sheep to monitor before making farm-wide changes. Document observations about animal behavior, feed consumption, and health markers.
  • Maintain a monthly log comparing feed efficiency, animal growth rates, and health observations between any animals receiving green propolis versus standard treatments. Track costs and any visible health differences to make informed decisions about broader adoption.

This research suggests green propolis may work as an alternative to salinomycin in sheep feed, but it is not yet established as a proven replacement. Farmers should not change their animal feeding practices based solely on this single study. Always consult with a veterinarian before making changes to livestock feed or management. This information is for educational purposes and does not constitute veterinary or medical advice. Individual results may vary based on specific farm conditions, animal breeds, and management practices. More research is needed to fully establish safety, effectiveness, and appropriate dosing guidelines.

This research translation is published by Gram Research, the science division of Gram, an AI-powered nutrition tracking app.

Source: Green propolis extract replaces salinomycin in the high-concentrate diet of sheep.Tropical animal health and production (2026). PubMed 41925932 | DOI